Giemsa Stain

About Giemsa Stain

Giemsa Stain is a laboratory reagent and stain for microscopy. A compound of eosin with methylene blue and its oxidation products. When blood films are stained using Giemsa stain, the nucleus and cytoplasm of white blood cells take on a characteristic blue or pink coloration. Giemsa stain functions by rearranging chromatin and inducing G bands producing a high quality stain for chromatin and the nuclear membrane. Giemsa stain is used to differentiate nuclear and/or cytoplasmic morphology of platelets, RBCs, WBCs, and parasites (1,2). The most dependable stain for blood parasites, particularly in thick films, is Giemsa stain containing azure B. Liquid stock is available commercially. The stain must be diluted for use with water buffered to pH 6.8 or 7.0 to 7.2, depending on the specific technique used.

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